Abstract
This paper presents the results of analysis of the genetic variability of seven Scots pine (Pinus sylvestris L.) populations in Serbia using SSR markers. Genomic DNA was isolated from seed tissue of all seven populations. The concentration of DNA samples was within the range of 1-4 mg/ml. Different PCR protocols were used depending on the type of SSR markers. The total number of fragments obtained by SSR analysis with 4 selected primers was 17 (only bands of strong and medium intensity were considered), of which 6 fragments were polymorphic (35.29%). In order to analyze the genetic similarity of the analyzed populations, graphs of correspondence analysis and UPGMA clusters were produced. By comparative analysis of the obtained dendrograms, the dependence of population genetic differentiation and spatial distance was observed, i.e. their isolation by natural barriers. The results indicate that in further research of interpopulation variability it is necessary, when graphically interpreting genetic distances, to use both methods of statistical analysis (UPGMA analysis and correspondence analysis).
Highlights
In Serbia, Scots pine (Pinus sylvestris L.) occupies about 26 600 ha and it occurs naturally on 7 527 ha
This paper presents the results of analysis of the genetic variability of seven Scots pine (Pinus sylvestris L.) populations in Serbia using SSR markers
The total number of fragments obtained by SSR analysis with 4 selected primers was 17, of which 6 fragments were polymorphic (35.29%)
Summary
In Serbia, Scots pine (Pinus sylvestris L.) occupies about 26 600 ha and it occurs naturally on 7 527 ha. The basis for the use of SSR markers in researching the genetic structure of Scots pine in seven selected populations in Serbia were the results of the analysis conducted by Karvonen and Savolainen (1993) and Liet et al (2005). The research in this paper was based on the analysis of the genetic structure of natural populations of Scots pine by forming a bulk sample for each population. This approach can reduce considerably the financial cost; the bulking of DNA samples results in the potential non-detection of rare alleles and the loss of information about the amount of heterozygosity within samples (Reif et al, 2005). Significant results in multidisciplinary research (genetic and phytocoenological) were obtained in the analysis of five populations of Scots pine in Serbia by combining these research methods (Lučić et al, 2011c)
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