Genetic diversity of Romanian Grey Steppe cattle based on milk protein polymorphism

Abstract

s / Journal of Biotechnology 185S (2014) S37–S125 S49 in the studied digestive organs. Results showed the possibility of using the “Ingo-2” in the practical medicine as a new nanosorbent. http://dx.doi.org/10.1016/j.jbiotec.2014.07.163 A new CT procedure for the study of the elbow in the dog Carla Murino1,∗, Giuseppina Mennonna1, Bruna Santangelo2, Francesco Lamagna2, Caterina Balestriere2, Leonardo Meomartino1 1 Radiology Veterinary Centre of University of Naples Federico II, Naples, Italy 2 Department of Veterinary Medicine and Animal Production of University of Naples Federico II, Naples, Italy E-mail address: carlamurino@gmail.com (C. Murino). Introduction: Previous described canine elbow-CT procedures need the head pulled laterally and caudally. This positioning is stressful for the neck and potentially dangerous in breeds prone to the Wobbler syndrome.Aim: Todevelop anewnon-stressful procedure for canine elbow-CT. Materials and methods: Prospectively, from January 2013, two elbow-CT procedures were used. The described procedure (DP): sternal recumbency with laterally and caudally pulled head and cranially extended forelimbs. The new procedure (NP): lateral recumbency, with forelimbs cranially pulled, very close one to each other and to the neck, in order to obtain the smallest field of view (FOV) and mean pixel dimension (MPD). CT procedures were compared for spatial resolution and presence of artefacts. Results: 18 elbows, from9dogs,were included.Mean FOV was: NP 126.6mm (±14.1); DP 103.3 (±5.16); mean MPD was: NP 0.25mm (±0.03); DP 0.21mm (±0.01). FOV and MPD were significantly larger inNP. Streak artefactswere absent in both procedures. Discussion and conclusion: compared to the DP, the NP has a significantly larger FOV and MPD, but a simpler and safer positioning, without streak artefacts. Furthermore, NP permits a more symmetrical positioning of the elbows and therefore to visualize them simultaneously. http://dx.doi.org/10.1016/j.jbiotec.2014.07.164 Genetic diversity of Romanian Grey Steppe cattle based on milk protein polymorphism Daniela Elena Ilie1,∗, Steofil Creanga2, Andrei Cristian Gradinaru3, Silviu Ionut Bors2, Dan Lucian Dascalu2, David Chirila4, Ada Cean1 1 Research and Development Station for Bovine, Arad, Romania 2 Research and Development Station for Bovine, Iasi, Romania 3 University of Agricultural Sciences and Veterinary Medicine of Iasi, Iasi, Romania 4 Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timisoara, Timisoara, Romania E-mail address: danailie@yahoo.com (D.E. Ilie). The aim of this study was to determine the polymorphism of the most important lacto-proteins in Romanian Grey Steppe breed, currently included in a genetic resource preservation program. DNA samples from55RomanianGrey Steppe individuals, including six bulls, were genotyped for kappa-casein ( -CN) and betalactoglobulin ( -LG) and only 50 samples for alpha-lactalbumin ( -LA), using the PCR-RFLP technique. The allele and genotype frequencies were calculated by direct counting, and the HardyWeinberg equilibrium was tested using the chi-square test ( 2). In addition, the homozygosity coefficient, based on homozygotic genotype frequencies,was statistically interpreted.AandBvariants were identified for -CN and -LG, whereas only the B allele was identified for -LA. The frequencies for A and B alleles were 0.464 and 0.536 for -CN, and 0.409 and 0.591 for -LG, respectively. The AB genotype was the most prevalent for both -CN and -LG (0.491 and 0.564), whereas the AA genotype displayed the lowest frequencies (0.218 and 0.127) for the same proteins. The results of the chi-square test for Hardy-Weinberg equilibrium showed that the investigated individuals are in genetic equilibrium. The calculated homozygosity coefficientwas 22.03%,which is representative for every Hardy-Weinberg population. Acknowledgements This work was financed through PNII-PCCA-120/2012 program. http://dx.doi.org/10.1016/j.jbiotec.2014.07.165 Using geographical information system for spatial evaluation of canine disc herniations Constantin Daraban1, Carla Murino2,∗, Giuseppe Marzatico2, Giuseppina Mennonna2, Gerardo Fatone2, Fabiana Micieli 2, Vasile Vulpe1, Leonardo Meomartino2 1 Faculty of Veterinary Medicine, Iasi, Romania 2 Department of Veterinary Medicine and Animal Production, Naples, Italy E-mail address: carlamurino@gmail.com (C. Murino). Background: Geographical Information System (GIS) is used in analyzinganddisplaying referenced information.GIS applicationshave never been reported in Veterinary Imaging. Aim: To develop a GISbased vertebral canal (VC) map useful for spatial evaluation of extruded disc herniation (EDH) in dogs. Methods: A GIS software (ArcGIS®) was used to create 2D and 3D maps in which VC surface was divided in polygons by lines representing the latitude and longitude. Depending on dislocation, EDH could be localized in one of the above regions. The actual dislocations of herniated disc material were assessed using a series of CT studies. Choropleth maps were realized based on frequency of EDH dislocation. Results: A cylindrical model of the VC with 78 latitudinal and 8 longitudinal lines dividing 624 polygons was created. Dislocation of EDH was assessed in 142 dogs with 177 EDH. Most of EDHs were located on the transitional regions (cervico-axial; cervico-thoracic; thoracolumbar; lumbo-sacral) at level of ventro-cranial and ventro-central polygons. Discussions: choropleth maps highlighted both the distribution and the dislocation pattern of EDH throughout the VC. Conclusion: GIS proved to be a valuable tool in analyzing dog’s EDH. Further studies are required for biomechanical analysis of EDH’s dislocation pattern. http://dx.doi.org/10.1016/j.jbiotec.2014.07.166