Abstract
Porcine epidemic diarrhea virus (PEDV) is one of the major enteric pathogens, causing severe enteric disease, resulting in enormous economic losses. The ORF3 gene encodes an accessory protein which is related to the infectivity and virulence of PEDV. In this study, 33 PEDV positive field samples were collected from Guangxi, from 2017 to 2019, and the genetic diversity of ORF3 was investigated. Thirty-eight strains of ORF3 were obtained, and these were composed of five strains of ORF3 named Guangxi naturally truncated strains that were 293 bp in length, with continuous deletions from 172 to 554 bp. The Guangxi naturally truncated strains encoded a truncated protein of 89 amino acids, which had clustered into a new group referred to as Group 3, and these might be involved in the variations of virulence. Three genotypes (G1-1 subgroup, G1-3 subgroup, and Group 3) existed simultaneously in Guangxi based on the genetic and evolutionary analysis of the ORF3 gene. The sequence information in the current study will hopefully facilitate the establishment of a diagnostic method that can differentiate the PEDV field stains. Continued surveillance will be useful for monitoring PEDV transmission. Differentiation of the ORF3 genes in PEDV field strains can help us to choose an appropriate PEDV vaccine candidate in the future and prevent outbreaks of PED more effectively.
Highlights
Porcine epidemic diarrhea virus (PEDV) is one of the major enteric pathogens currently threatening the swine population worldwide [1]
PED is a highly contagious intestinal infectious disease in pigs caused by PEDV, which has seriously affected the development of the pig breeding industry and caused significant economic losses
It has recently been proposed that PEDV ORF3 plays a role in regulating PEDV replication and pathogenesis [1, 20, 30]
Summary
Porcine epidemic diarrhea virus (PEDV) is one of the major enteric pathogens currently threatening the swine population worldwide [1]. Pigs infected with PEDV cause severe enteric diseases with a high mortality rate in suckling piglets, resulting in tremendous economic losses [2,3,4,5,6]. PEDV first emerged in Europe in the 1970s and spread across Europe and into Asia [10]. In China, outbreaks of PEDV have been observed on most swine breeding farms since late 2010 [6, 8, 11]. PEDV has rapidly spread across 34 states of America, Canada, and has returned to devastate the swine industry in Asia after being diagnosed in the USA in April 2013 [12, 13]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
