Abstract
This study was carried out to assess the genetic diversity in 48 genotypes and accessions using eight Amplified Fragment Length Polymorphism (AFLP) primer-pairs. The eight selected AFLP primer-pairs generated a total of 150 polymorphic loci. Using the generated AFLP data, the Un-Weighted Pair Group Method with Arithmetic Average (UPGMA) ordered the genotypes into six groups based on Dice similarity coefficient. The range in taxonomic distance was from 0.23 to 1.0. Each cluster was found to have genotypes and accessions from different regions and climate and sometimes different continents. The size range of the loci ranged from 87-662 bp. Great variation between the genotypes and accessions in the different cluster could be of high value as the genetically diverse okra genotypes represent a potentially valuable source for improved pathogen and pest resistance.
Highlights
Okra is a member of the malvaceae family, which includes fiber crops such as cotton (Gossypium spp) and kenaf (Hibiscus cannabianus)
This study was carried out to assess the genetic diversity in 48 genotypes and accessions using eight Amplified Fragment Length Polymorphism (AFLP) primer-pairs
Using the generated AFLP data, the Un-Weighted Pair Group Method with Arithmetic Average (UPGMA) ordered the genotypes into six groups based on Dice similarity coefficient
Summary
Okra is a member of the malvaceae family, which includes fiber crops such as cotton (Gossypium spp) and kenaf (Hibiscus cannabianus). (Borssum Waalkes, 1966; Bates, 1968). The genus Abelmoschus comprises nine species (IBPGR, 1990). It is a traditional vegetable crop in many tropical, subtropical and Mediterranean countries. The origin of okra remains unclear but centers of genetic diversity include West Africa, India and Southeast Asia (Charrier, 1984; Hamon and Van Sloten, 1989). Van Borssum-Waalkes distinguished only six species: three cultivated (A. moschantus, A. manihot and A. esculentus) and three wild (A. ficuleus, A. crinitus and A. angulosus). Kundu and Biswas (1973; Terell and Winters, 1974) distinguished the genus Abelmoschus from Hibiscus. Okra seeds have gained much interest as a new oil and protein source (Düzyaman, 1997)
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