Genetic diversity of microorganisms capable of degrading diesel as a pool of bioremediation

Abstract

The ability to degrade the diesel by bacterial species (Micrococcus spp., Staphylococcus spp., and Celibiosococcus spp.) as a sole source of hydrocarbon was studied in the present work. In laboratory by identifying and assessing the potential of hydrocarbon degrader by micro titer plate method, the best diesel degrading bacteria was isolated. The similarity trait among the 25 bacterial isolates was also assessed. All the bacterial species utilized the hydrocarbons as sole carbon and energy sources showing increases in cell number and optical density with decreases in pH of the culture media. This study shown that Micrococcus spp., has maximum ability to degrade diesel. The banding pattern of RAPD showed that all the isolates share some similarity between them RAPD was chosen as it has been shown to be effective sub typing method for several other species.