Abstract
Lima bean (Phaseolus lunatus L.) is one of the important legume vegetables in Indonesia. However, genetic information for these plants is still minimalized, especially on Timor Island. This study aims to analyze the genetic diversity of lima beans from Timor Island based on ISSR molecular markers. A total of 4 accessions of lima beans were analyzed using 3 ISSR primers to produce 15 polymorphic bands with an average of 68.18% polymorphism. The cluster analysis results use the Unweighted Pair Group Methods using Arithmetic averages (UPGMA) method to create a dendrogram that produces two main clusters. There were plain seed and pattern seed group with a similarity coefficient of 0.52. These results indicated that the genetic variation of the lima beans from Timor Island was high. Moreover, the result provides a sutable method for evaluating the genetic diversity of lima beans using the ISSR marker and important information of future lima bean breeding programs.
Highlights
Floribunda merupakan organ resmi Penggalang Taksonomi Tumbuhan Indonesia, diterbitkan dua kali setahun dan menerbitkan makalah dalam bahasa Indonesia dan Inggris mengenai pelbagai gatra sistematika keanekaragaman flora Malesia pada umumnya dan Indonesia pada khususnya yang berasal dari hasil penelitian, pengamatan lapangan, pengalaman pribadi, telaahan bergagasan, dan tinjauan kritis
This study aims to analyze the genetic diversity of lima beans from Timor Island based on Inter Simple Sequence Repeat (ISSR) molecular markers
Materials Materials used for this study was lima bean accessions collected from the Timor island aged 34 weeks old (Table 1)
Summary
Floribunda merupakan organ resmi Penggalang Taksonomi Tumbuhan Indonesia, diterbitkan dua kali setahun dan menerbitkan makalah dalam bahasa Indonesia dan Inggris mengenai pelbagai gatra sistematika keanekaragaman flora Malesia pada umumnya dan Indonesia pada khususnya yang berasal dari hasil penelitian, pengamatan lapangan, pengalaman pribadi, telaahan bergagasan, dan tinjauan kritis. Jenis tulisan Makalah lengkap memuat hasil penelitian floristik, revisi, atau monografi unsur-unsur flora Malesia. Komunikasi pendek mencakup laporan kemajuan kegiatan penelitian, pengembangan dan rekayasa keanekaragaman flora Malesia yang perlu segera dikomunikasikan. Tulisan lain meliputi obituari tokoh keanekaragaman flora, tinjauan kritis bergagasan, telaahan serta pembahasan persoalan aktual seputar kegiatan penelitian, pengembangan dan rekayasa tetumbuhan Indonesia, serta timbangan buku akan dimuat berdasarkan undangan. Pedoman Umum Ejaan yang Disempurnakan, Pedoman Umum Pembentukan Istilah, Kamus Besar Bahasa Indonesia, serta kamus-kamus istilah yang dikeluarkan Pusat Bahasa. Bahasa Inggris yang dipakai adalah the Queen
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