Genetic diversity of ivory shell (Babylonia areolata) in Taiwan and identification of species using DNA-based assays

Abstract

For better traceability of seafood products in Taiwan, we need to effectively test product quality during the processes of identification of seafood species. The aim of this study was to analyse gene diversity and the methods of identification of high-value seafood, ivory shell (Babylonia areolata), on the Penghu Island in Taiwan using molecular marker technology and to build a relevant molecular database. Thirty-three samples of B. areolata and the samples of 5 other Babylonia species, including Babylonia feicheni, Babylonia spirata spirata, Babylonia perforata and Babylonia formosae formosae, from cultivation and from the wild were tested using the inter-simple sequence repeat (ISSR) method, mitochondrial DNA analysis, PCR-single-strand conformation polymorphism (PCR-SSCP) and PCR-denaturing gradient gel electrophoresis (PCR-DGGE). The results showed that the primers ISSR3, ISSR7 and ISSR13 of the ISSR method and cytochrome C oxidase subunit 1 (coxI) gene analysis have a good discriminatory power in inter-species and intra-species tests. In conclusion, ISSR, PCR-DGGE and PCR-SSCP with coxI analysis can be used for the screening and identification of B. areolata species. Furthermore, these molecular methods could be useful for the identification of other types of seafood.