Abstract
Empoasca flavescens Fabricius (Homoptera: Cicadellidae), is an emerging insect pest of tea, Camelia sinensis L. (Theaceae), in sub-Himalayan region of West Bengal. Use of synthetic insecticides in tea plantations against insect pests may have resulted in development of resistant E. flavescens populations. This was tested by recording genetic variations in the populations of E. flavescens collected from three different locations of North Bengal. Three populations were studied using RAPD-PCR. Polymorphism in populations was assayed using eight random primers resulting in amplification of 53 loci. The polymorphic loci recorded were 83.02, 84.92 and 88.68 % in Darjeeling hill, Terai and the Dooars populations, respectively. The genetic distance between Darjeeling hill and Terai, Darjeeling hill and the Dooars populations were found to be 0.0255 and 0.0332, respectively. UPGMA dendrogram based on the ‘Nei’s (Genetics 89: 583–590, 1978) genetic distance method’ showed greater genetic distance of Darjeeling population from Terai as compared to Dooars populations.
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