Genetic Diversity of Botrytis Cinerea Which Caused Gray Mold Disease in Strawberry and Some Other Vegetable Plants

Abstract

The study aimed to isolate and identification the fungus Botrytis cinerea that caused gray mold disease, depending on its morphological and molecular characterizations. The results of isolation on PDA showed that 75 pure isolates were obtained from The pathogenic fungus B. cinerea is distributed as follows: Strawberry (45) isolates, eggplant (11) isolates, squash (3) isolates, cucumber (3) isolates, pepper (9) isolates, and tomato (4) isolates. DNA was extracted from 17 isolates of pathogenic fungus B. cinerea, the results of electrophoresis on 2% agarose gel, which included 17 isolates distributed in crops (strawberry 11 isolates, eggplant 4 isolates, squash and tomato one isolate each) was detected at 550 base pair(bp) of all the isolates using ITS1 and ITS4 primers. The results of detection and investigation of genetic variation of pathogenic Fungi isolates were clarified by determining the degree of kinship and drawing a phylogenetic tree An evolutionary and relationship tree was drawn using Snap Gene Viewer, When performing Allayment analysis of the isolates, the results showed that the isolates bearing the symbols (S3,S2,S1) isolated from strawberry fruits were identical to the SMGM003 strain registered in South Korea with a percentage of 100%, and isolates (S6,S5,S4) isolated from strawberry fruits are identical to the strain WMG recorded in South Korea, and isolates (S10,S9,S8) isolated from strawberry fruits are identical to the strain CIM13704 registered in China, and isolates (S11) isolated from strawberry fruits.