Genetic Diversity of Bile Salt Hydrolases Among Human Intestinal Bifidobacteria

Piotr Jarocki,Zdzisław Targoński

doi:10.1007/s00284-013-0362-1

Piotr Jarocki, Zdzisław Targoński

Open Access

https://doi.org/10.1007/s00284-013-0362-1

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Journal: Current Microbiology	Publication Date: Apr 17, 2013
Citations: 52	License type: CC BY 2.0

Affiliation: University of Life Sciences in Lublin

Abstract

This study analyzes the application of degenerative primers for the screening of bile salt hydrolase-encoding genes (bsh) in various intestinal bifidobacteria. In the first stage, the design and evaluation of the universal PCR primers for amplifying the partial coding sequence of bile salt hydrolase in bifidobacteria were performed. The amplified bsh gene fragments were sequenced and the obtained sequences were compared to the bsh genes present in GenBank. The determined results showed the utility of the designed PCR primers for the amplification of partial gene encoding bile salt hydrolase in different intestinal bifidobacteria. Moreover, sequence analysis revealed that bile salt hydrolase-encoding genes may be used as valuable molecular markers for phylogenetic studies and identification of even closely related members of the genus Bifidobacterium.

Highlights

Members of the genus Bifidobacterium are anaerobic Gram-positive Actinobacteria, which are commonly found in the gastrointestinal tract (GIT) of humans and animals [21, 22]
We present the application of degenerative primers for the screening of bsh genes in various human intestinal bifidobacteria
The obtained results demonstrated the utility of these primers for the amplification of bsh fragments from different intestinal bifidobacterial species (Fig. 2)

Summary

Introduction

Members of the genus Bifidobacterium are anaerobic Gram-positive Actinobacteria, which are commonly found in the gastrointestinal tract (GIT) of humans and animals [21, 22]. Numerous studies have shown that the presence of the bifidobacteria in the human gut is associated with many health-promoting effects, such as immunomodulation, prevention of diarrhea, reduction of pathogens and toxic compounds, decrease of lactose intolerance, and stabilization of the state of the intestine [2, 3, 6, 11]. Because of this, they are frequently used as food additives in the dairy industry and therapeutic agents in some probiotic pharmaceuticals. The expression of bile salt hydrolase genes was tested by the RT-PCR detection of internal fragments of the analyzed bsh genes

Materials and Methods

Results and Discussion

Conclusions