Abstract
The purpose of this study was to apply autosomal STRs and X-STRs to confirm paternity and sibship in a sample of 315 female twins and triplets from the region of Murcia in Spain. X-STR amplification was performed using GEP-ISFG X-STR decaplex primers. Deviations from Hardy-Weinberg equilibrium were not observed at any loci. Acceptable levels of power of discrimination and mean exclusion chance were determined. The highest locus diversity and power of discrimination was determined for loci GATA172D05 and GATA31E08. Combined power of discrimination was 0.9999999997 (females) 0.999998548 (males). Mean exclusion chance was 0.999993172 (trios) and 0.999708265 (duos). Pairwise genetic distance (Fst) of X-STR frequencies suggest that Murcia can be closely grouped to other Iberian populations, and genetically furthest from Pas Valley, Navarra and Basque Country.
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