Abstract

Genetic diversity in 36 populations of wild taxa of theBrassica oleraceaL. group (2n=18) and two cultivated forms was studied using isozyme variation at 11 loci for five enzyme systems (IDH, 6-PGD, PGM, PGI, MDH). Mean values for the percentage of polymorphic loci and expected heterozygosity were 54% and 0.224, respectively. Statistically significant differences among allele frequencies were found with the 6-PGD isozyme system. Intrapopulational genetic diversity was 67% while interpopulational genetic diversity was only 33%. The dendrogram obtained, using genetic distances among taxa, showed three different groups. With the exception ofB. incana,they agree to the already accepted relationships among the 14 taxa studied: the West Mediterranean group, withB. oleracea, B. alboglabra, B. bourgeauiandB. incana; another group of species growing in the central Mediterranean area, which includesB. villosa, B. villosasubsp.drepanensis, B. rupestris, B. macrocarpa(the four taxa together withB. incanaare considered theB. rupestrisgroup) andB. montana; and finally the Aegean group, which includes the three subspecies ofB. cretica.Clearly separated wereB. insularisandB. hilarionis, showing the maximum genetic distance. Separate dendrograms were also obtained forB. oleracea, B. montana, B. creticaandB. rupestrisgroup, and genetic diversity parameters were estimated. Genetic distances amongB. oleraceapopulations are in the same range as populations of theB. creticasubspecies. Highest genetic distances were found among populations of theB. rupestrisgroup.

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