Genetic diversity in sugar apple (Annona squamosa L.) by using RAPD markers

João Filipi Rodrigues Guimarães,Wagner Vendrame,Glaucia Bethania Rocha Moreira,Márcia Regina Costa,Silvia Nietsche,Marlon Cristian Toledo Pereira

doi:10.1590/s0034-737x2013000300017

João Filipi Rodrigues Guimarães, Wagner Vendrame + Show 4 more

Open Access

https://doi.org/10.1590/s0034-737x2013000300017

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Abstract

Genetic diversity in a collection of 64 sugar apple accessions collected from different municipalities in northern Minas Gerais was assessed by RAPD analysis. Using 20 selected RAPD primers 167 fragments were generated, of which 48 were polymorphic (28.7%) producing an average of 2.4 polymorphic fragments per primer. Low percentage of polymorphism (< 29%) was observed by using the set of primers indicating low level of genetic variation among the 64 accessions evaluated. Genetic relationships were estimated using Jaccard's coefficient of similarity. Accessions from different municipalities clustered together indicating no correlation between molecular grouping and geographical origin. The dendrogram revealed five clusters. The first cluster grouped C19 and G29 accessions collected from the municipalities of Verdelândia and Monte Azul, respectively. The second cluster grouped G16 and B11 accessions collected from the municipalities of Monte Azul and Coração de Jesus, respectively. The remaining accessions were grouped in three clusters, with 8, 15 and 37 accessions, respectively. In summary, RAPD showed a low percentage of polymorphism in the germplasm collection.

Highlights

In Brazil, sugar apple (Annona squamosa L.) is an important fruit crop grown commercially in semiarid regions
Brazil has the largest number of sugar apple accessions (92); a systematic effort to evaluate these accessions will be fundamental to improve the species (IPGRI, 2000)
The objective of the present study was to assess the genetic diversity in 64 accessions of sugar apple germplasm by using RAPD markers

Summary

Introduction

In Brazil, sugar apple (Annona squamosa L.) is an important fruit crop grown commercially in semiarid regions. Annonaceae family presents a large intra and interspecific variability offering an ample scope for studying genetic variation. Despite this great variability, germplasm banks that contain Annona spp., mainly Annona squamosa, are rare throughout the world, which is a limiting factor for selecting and crossing among elite cultivars (Pinto et al, 2005). Molecular markers have provided a powerful tool for proper characterization of germplasm diversity (Williams et al, 1990). PCR-based molecular techniques provide a variety of DNA markers for diversity analysis (Newbury & Ford-Lloyd, 1993), identification of cultivars (Matsumoto & Fukui, 1996), gene localization, and construction of linkage maps (Giovanni et al, 2004). RAPD markers have been used for diversity analysis in cacao (Leal et al, 2008), banana (Souza et al, 2008) and, sugar apple (Bharad et al, 2009, Ahmad et al, 2010)

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