Abstract

The current study was undertaken to estimate the morphological and molecular diversity present among the 51 accessions of Indian opium poppy germplasm using Mahalanobis D2 and SCoT (Start Codon Targeted Polymorphism) marker analyses, respectively. A good range of morphological variations were observed among the accessions. The accessions were clumped into nine clusters and the morphological diversity recorded was 69%. Clusters VII and IX showed the maximum inter-cluster distance (117.97) whereas it was found to be minimum (21.53) in case of clusters II and III. The morphological trait, seed yield per capsule, contributed maximum (17.30%) towards the genetic divergence followed by thebaine content (14.56%) and papaverine content (14.06%). In contrast to the morphological diversity, genetic diversity at molecular level was found to be limited (33%), although successfully detected by utilizing DNA markers targeting coding regions of genome (SCoT markers). The outcome of the study has prospects in identification of lines with desirable traits to be utilized in future breeding programmes.

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