Abstract
Received: 2018-05-07 | Accepted: 2018-05-14 | Available online: 2018-11-26 https://doi.org/10.15414/afz.2018.21.04.190-193 The aim of the present study was to analyse the genetic diversity of the endangered horse breeds kept in the Czech Republic. A set of 13 microsatellites was used for genotyping 349 Silesian Norikers, 397 Norikers, 552 Czech-Moravian Belgian horses, 271 Old Kladrubers (175 greys, 95 blacks) and 241 Hucul horses. The proportion of obtained heterozygosity indicates no major loss of genetic diversity within analyzed breeds. The Wright’s F ST and genetic distances indicated genetic segregation of both colour varieties of the Old Kladruber breed and small genetic distances between draft horse breeds. Moreover, the membership probability outputs showed that the frequencies of alleles varied across the three main regions. First region is represented by draft horse breeds, second region is represented by Old Kladruber horse and the last is represented by Hucul breed. The study provides data and information utilizable in the management of conservation programs in order to reduce inbreeding and to minimize loss of genetic variability. Keywords : admixture, endangered breeds, horse, loss of genetic diversity References Delgado J.F., De Andres N., Valera M., Gutierrez J.P., Cervantes I. (2014) Assessment of population structure depending on breeding objectives in Spanish Arabian horse by genealogical and molecular information. Livestock Science, 168, 9–16. DOI: https://dx.doi.org/10.1016/j.livsci.2014.07.012 Jombart T., Ahmed I. (2011) adegenet 1.3-1: new tools for the analysis of genome-wide SNP data. Bioinformatics, 27, 3070–3071. DOI: https://dx.doi.org/10.1093/bioinformatics/btr521 Jombart T., Collins C. (2015): A tutorial for diskriminant analysis of principal components (DAPC) using adegenet 2.0.0. MRC Centre for Outbreak Analysis and Modelling. [Online] London: Imperial College London. Available at: http://adegenet.rforge. r-project.org/files/tutorial-dapc.pdf [accessed 20 November 2017]. Kasarda R., Vostry L., Moravcikova N., Vostra-Vydrova H., Dovc P., Kadlecik O. (2016) Detailed insight into genetic diversity of the Old Kladruber horse substructure in comparison to the Lipizzan breed. Acta Agriculturae Scandinavica, Section A – Animal Science, 66, 67–74. DOI: https://dx.doi.org/10.1080/09064702.2016.1249400 Peakall, R. and Smouse P.E. (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for taching and research-an update. Bioinformatics 28, 2537-2539. DOI: https://dx.doi.org/10.1093/bioinformatics/bts460 Szwaczkowski T., Gregula-Kania M., Stachurska A., Borowska A., Jaworski Z., Gruszecki T.M. (2016) Interand intra-genetic diversity in the Polish Konik horse: implications for the conservation program. Canadian Journal of Animal Science, 96 (4), 570–580. DOI: https://dx.doi.org/10.1139/cjas-2015-0173
Highlights
Genetic diversity studies in domestic animals focus on evaluating genetic variation within and across breeds mainly for conservation purposes
In the case of missing or incomplete pedigree, it would be better to use molecular information to characterize a population; the molecular information indicates the additional relatedness between animals appearing as founders in the pedigree (Delgado et al 2014).The aim of this study was to analysis of genetic diversity within and between five endangered Czech horse breeds
The higher values of NA and number of alleles (Ne) were estimated for H breed
Summary
Genetic diversity studies in domestic animals focus on evaluating genetic variation within and across breeds mainly for conservation purposes. An effective management of farm animal resources requires comprehensive knowledge of the breeds’ characteristics including data on population size and structure and within and between breed genetic diversity. The knowledge of genetic characterization and genetic structure is the first step in breed conservation and may have implications for future breeding strategies and management plans. In the case of missing or incomplete pedigree, it would be better to use molecular information to characterize a population; the molecular information indicates the additional relatedness between animals appearing as founders in the pedigree (Delgado et al 2014).The aim of this study was to analysis of genetic diversity within and between five endangered Czech horse breeds
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