Abstract
Genetic diversity among 12 Egyptian bottle gourd genotypes was assessed using 11 ISSR molecular markers. The ISSR analysis generated 76 amplicons in a size range of 170–1600 bp with maximum (9) polymorphic amplicons by UBC 811. The high degree of polymorphism and PIC values greater than 0.50 indicate that 844-B, HB 10, UBC 807 and UBC 825 markers could be useful for rapid fingerprinting of many bottle gourd genotypes. Parameters of genetic diversity and its partitioning were estimated among genotypes. Various parameters namely, observed heterozygosity, effective number of alleles per locus, Shannon's index, expected heterozygosity, and represents percentage of polymorphic band were estimated. The genetic analysis demonstrated that Egyptian bottle gourd genotypes keep relatively high genetic diversity. Cluster analysis (UPGMA) clustered all genotypes into three main clusters based on the ISSR data. Overall, the results indicated that fingerprinting using ISSR markers might be an efficient tool in future breeding programs for varietal identification and assessing genetic diversity within the Egyptian bottle gourd genotypes. The high genetic variability among studied genotypes would be beneficial for breeding and conservation programs.
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