Genetic diversity in conventional and synthetic wheats with drought and salinity tolerance based on AFLP

Abstract

Genetic diversity among 14 drought tolerance (drought accessions) and 27 salinity tolerance (salinity accesions) related conventional and synthetic wheat (Triticum sp.) accessions containing different sources of the D genome was assessed using amplified fragment length polymorphism (AFLP). The wheat accessions were analyzed with 20 EcoRI/MseI primer combinations. Among 918 fragments scored, 368 were polymorphic across all 41 wheat accessions, 348 were polymorphic among the drought accessions and 310 were polymorphic among the salinity accessions. Similarity coefficients among all accessions based on Jaccard’s coefficient ranged from 0.18 to 0.92 with an average of 0.53 ± 0.01; among drought accessions, from 0.16 to 0.79 with an average of 0.43 ± 0.02; and among salinity accessions, from 0.16 to 0.92 with an average of 0.57 ± 0.01. Polymorphic information content (PIC) among all accessions ranged from 0.05 to 0.50 with an average PIC of 0.30 ± 0.01; among drought accessions, from 0.13 to 0.50 with an average PIC of 0.37 ± 0.01; and among salinity accessions, from 0.07 to 0.50 with an average PIC of 0.29 ± 0.01. Cluster and principal component analysis showed distinct groups of accessions both within drought and salinity entries. These accessions possess a substantial amount of genetic diversity and would be very valuable materials for breeding wheat with drought and salinity tolerance. Key words: AFLP, DNA fingerprinting, genetic diversity, synthetic wheat