Genetic diversity in Banija spotted pig: pedigree and microsatellite analyses

Abstract

Genetic diversity and breed structure of Banija spotted pig (BS) was analyzed trough 721 pedigree records and polymorphism of 23 microsatellites on 30 BS pigs. Two phylogenetic trees were constructed from microsatellite and pedigree information. The inbreeding coefficient obtained by microsatellite markers was 5.6%, while the inbreeding coefficient from pedigree analysis was 3.68%. The rate of inbreeding per generation was 1.74% and effective population size was 28.81. In phylogenetic analyses, relationship coefficients and genetic distances between individuals were calculated using microsatellite and pedigree data. Phylogenetic trees from microsatellite markers and pedigree corresponded well to each other and showed consistency between microsatellite and pedigree information. From the data obtained by microsatellite markers and pedigree, two subpopulations can be observed. The existence of two subpopulations can be explained by two different paths of breed genesis. Thus, future work in the conservation process should include methods such as optimal contribution selection including factorial mating, in order to make genetic progress and control the rate of inbreeding.