GENETIC DIVERSITY IN AMBURANA (Amburana cearensis) ACCESSIONS: HIERARCHICAL AND OPTIMIZATION METHODS

Ana Valéria Vieira De Souza,Jackson Rafael De Sá Carvalho,Ana Carolina Silva Siquieroli,Herick Fernando De Jesus Silva,Gabriel Mascarenhas Maciel,José Magno Queiroz Luz,Evelyn Sophia Silva Costa,Cristina Soares De Souza

doi:10.1590/1806-908820210000008

Abstract

ABSTRACT The evaluation of accessions in a germplasm bank is essential for determining the potential parents in conservation programs, especially for native trees. This study aimed to determine the genetic diversity among 68 Amburana cearensis genotypes from different locations in the state of Pernambuco, Brazil. Their genetic patterns were evaluated by Inter Simple Sequence Repeat (ISSR) molecular markers and genetic divergence was evaluated through multivariate analyses using different clustering methods. The optimization method used (Tocher) was in agreement with all the hierarchical models used, in which clustering of the genotypes occurred similarly, specifically for the accession BB116, which is an important genetic material to be preserved and studied. Among the various hierarchical methods applied, the Average Linkage method exhibited higher discrimination power, allowing identification of a larger number of divergent groups, thus implying wide genetic diversity among A. cearensis accessions.

Highlights

This study aimed to determine the genetic diversity among 68 Amburana cearensis genotypes from different locations in the state of Pernambuco, Brazil
Their genetic patterns were evaluated by Inter Simple Sequence Repeat (ISSR) molecular markers and genetic divergence was evaluated through multivariate analyses using different clustering methods
The propagative materials used came from six populations in the following locations, with their respective abbreviations: Jutaí (J), Rocinha (RO), Lajedo (LA), Barra Bonita (BB), Caiçara (CA), and Baixa do Juazeiro (BJ), all situated in the state of Pernambuco, Brazil (Figure 1)

Summary

Introduction

C. Smith (Fabaceae) or amburana is a tree native to the Caatinga (Brazilian xeric shrubland and thorn forest) with multiple uses, such as lumber; medicinal uses, attributed to coumarin, the active ingredient present in bark and roots; ornamental use; reforestation in agroforestry systems (Pimentel and Guerra, 2015); and forage. Smith (Fabaceae) or amburana is a tree native to the Caatinga (Brazilian xeric shrubland and thorn forest) with multiple uses, such as lumber; medicinal uses, attributed to coumarin, the active ingredient present in bark and roots; ornamental use; reforestation in agroforestry systems (Pimentel and Guerra, 2015); and forage It is important for bee raising as it provides nectar in the dry season of the year (Silva et al, 2014). It has sexual propagation and a considerable percentage of germination (Angelim et al, 2007)

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