Genetic diversity between large white and nigerian indigenous breed of swine using polyacrylamide gel electrophoresis (PAGE).

Hm Ndofor-Foleng,Og Iloghalu,Mo Onodugo,Ag Ezekwe

doi:10.4314/as.v13i3.5

Abstract

Sixty-two pigs (32 large white pigs and 32 Nigerian indigenous pigs were used in this study. Biochemical techniques were used in analyzing the blood serum samples obtained from the pigs using Polyacrylamide Gel Electrophoresis. A total of 14 loci were scored for the Nigerian Indigenous pigs and 10 for the Large White breed. The results of the molecular characterization with SDS-PAGE were analysed using PAST Package to determine genetic similarity coefficient and construct phylogenetic dendrogram. The within breed comparism of Nigerian indigenous pigs showed 62% similarity while the Large White breeds showed 65% similarity. For the between-breed comparism, the Nigerian indigenous breeds and the Large White breeds were 48% similar. The moderate genetic similarity observed in the Nigerian indigenous pigs and the Large White pigs indicates a reasonable level of genetic dilution in both breeds. Keywords : Electrophoresis, Genetic diversity, Pig, Polymorphism,

Highlights

Indigenous pigs have played an important role in smallholder farms and local populations for a long time (Mutua et al, 2011)
The local breeds were purchased from local pig farmers within the middle belt of Nigeria (Gboko) while the Large White breed was obtained from the piggery unit, University Of Nigeria Teaching and Research Farm
Plate 1 and 2contains 10 lanes (1-10) each of different pig serum samples obtained from large white breed and Nigerian indigenous breed

Summary

Introduction

Indigenous pigs have played an important role in smallholder farms and local populations for a long time (Mutua et al, 2011). The conservation of indigenous and exotic pigs in Nigeria as a genetic resource and vital components within the livestock sectorhave some challenges. Genetic diversity is an asset for the future development of livestock production in Nigeria. The genetic characterization of local pigs in Nigeria should be the first step in considering the sustainable management or conservation of a particular population. The genetic characterization of the domestic animals is part of the Food and Agricultural Organization global strategy for the management of farms (FAO, 2012). This strategy places a strong emphasis on the use of molecular methods to assist the conservation of endangered breeds and to determine the genetic status of breeds. Electrophoresis is one of the methods for the study of genetic diversity and has severally been used to establish the genetic distances among breeds and/or populations (Akinyemi et al, 2014)

Methods

Results

Conclusion