Abstract
In the present investigations, Randomly Amplified Polymorphic DNA (RAPD) technique has been used to detect polymorphism of Mucuna atropurpurea DC. in Southern Western Ghats, Tamil Nadu, India. The genomic DNA was extracted from the fresh and young healthy leaves of the selected species using modified CTAB method which successfully produced a good purity and quality of DNA. Alleles of various sizes ranging from 100 – 800bp were amplified. The average genetic distance range was 67% – 91%. Phylogenetic relationships among these accessions based upon DNA analysis were studied through dendrogram analysis. All the accessions were clustered in to two groups. In this cluster the Kothaiyar and Saduragiri accessions were found most distantly related to each other.
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