Genetic diversity assessment in sorghum accessions using qualitative morphological and amplified fragment length polymorphism markers

Abe Shegro Gerrano,Angeline Van Biljon,Maryke Tine Labuschagne,Nemera Geleta Shargie

doi:10.1590/0103-9016-2013-0251

Abe Shegro Gerrano, Angeline Van Biljon + Show 2 more

Open Access

https://doi.org/10.1590/0103-9016-2013-0251

Copy DOI

Abstract

Qualitative morphological and amplified fragment length polymorphism (AFLP) markers were compared for assessment of genetic diversity. Nine qualitative morphological traits were recorded to compare genetic relationships among 17 sorghum accessions with information derived from six AFLP primer combinations analysis. The mean morphological genetic similarity was lower in comparison to similarity computed using AFLP markers. Genetic similarity measured by AFLP markers was similar within the Ethiopian and South African material, as well as between South African and Ethiopian material. Morphological similarity was much higher in the Ethiopian material than in the South African material, indicating that the genotypes were related. The two techniques described genetic variability in different ways. Dendrogram generated from the morphological data matrix separated accession 216737 as being genetically distinct from the rest of the accessions. Accessions M101 and 97MW6127 were the most dissimilar accessions based on AFLP data.

Highlights

Information on genetic diversity has been successfully used for efficient germplasm management and utilisation (Frankel, 1989; Blakeney, 2002), genetic fingerprinting and genotype selection (IBPGR, 1993; Bucheyeki et al, 2009; FAO, 1998; Engles et al, 2002) in crop improvement
Qualitative morphological and amplified fragment length polymorphism (AFLP) markers were compared for assessment of genetic diversity
Nine qualitative morphological traits were recorded to compare genetic relationships among 17 sorghum accessions with information derived from six AFLP primer combinations analysis

Summary

Introduction

Information on genetic diversity has been successfully used for efficient germplasm management and utilisation (Frankel, 1989; Blakeney, 2002), genetic fingerprinting and genotype selection (IBPGR, 1993; Bucheyeki et al, 2009; FAO, 1998; Engles et al, 2002) in crop improvement. Morphological descriptors, biochemical and molecular markers are the methods used for measuring genetic diversity among crop species (Geleta and Labuschagne, 2005; Mehmood et al, 2008). Molecular markers are invaluable for understanding the genetic make-up of agricultural crops They differ from morphological traits in that they usually occur in greater numbers, they can be distinguished without relying on the complete development of the plant and their expression is not altered by the environment (Jeya Prakash et al, 2006; Tabbasam et al, 2006; Mehmood et al, 2008; Abu Assar et al, 2009)

Objectives

Methods

Results