Abstract
BackgroundA core collection is a subset of an entire collection that represents as much of the genetic diversity of the entire collection as possible. The establishment of a core collection for crops is practical for efficient management and use of germplasm. However, the establishment of a core collection of mushrooms is still in its infancy, and no established core collection of the economically important species Flammulina velutipes has been reported.ResultsWe established the first core collection of F. velutipes, containing 32 strains based on 81 genetically different F. veltuipes strains. The allele retention proportion of the core collection for the entire collection was 100%. Moreover, the genetic diversity parameters (the effective number of alleles, Nei’s expected heterozygosity, the number of observed heterozygosity, and Shannon’s information index) of the core collection showed no significant differences from the entire collection (p > 0.01). Thus, the core collection is representative of the genetic diversity of the entire collection. Genetic structure analyses of the core collection revealed that the 32 strains could be clustered into 6 groups, among which groups 1 to 3 were cultivars and groups 4 to 6 were wild strains. The wild strains from different locations harbor their own specific alleles, and were clustered stringently in accordance with their geographic origins. Genetic diversity analyses of the core collection revealed that the wild strains possessed greater genetic diversity than the cultivars.ConclusionWe established the first core collection of F. velutipes in China, which is an important platform for efficient breeding of this mushroom in the future. In addition, the wild strains in the core collection possess favorable agronomic characters and produce unique bioactive compounds, adding value to the platform. More attention should be paid to wild strains in further strain breeding.
Highlights
A core collection is a subset of an entire collection that represents as much of the genetic diversity of the entire collection as possible
The t-tests of the mean genetic diversity parameters (Ae, H, Ho, I) of the core collection and the entire collection were non-significant (p > 0.01) (Table 2), which reveals that the genetic diversity of the core collection has no significant difference from that of the entire collection
Based on an unweighted pair group method with arithmetic mean (UPGMA) dendrogram of the core collection and the entire collection (Fig. 1), the strains in different groups of the entire collection were uniformly selected for the core collection
Summary
A core collection is a subset of an entire collection that represents as much of the genetic diversity of the entire collection as possible. The establishment of a core collection for crops is practical for efficient management and use of germplasm. A core collection is a subset of accessions that presents the maximum possible genetic diversity contained in an entire collection with minimum redundancy [1, 2]. The establishment of a core collection for crops is practical for efficient management of germplasm. Core collections of most major food crops, such as Oryza sativa, Zea. A core collection is traditionally constructed based on morphological and agronomic characters using different strategies, such as the constant allocation (C) strategy, the logarithm (L) strategy, the proportional allocation (P) strategy, and the random sampling (R) strategy [2, 8,9,10]. Liu et al Hereditas (2018) 155:3 Strain Cultivar/wild.
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