Abstract
DNA polymorphism in the cultivar species; Cucumis sativus L., C. melo L. and Benincasa hispida Cogn. of subtribe Cucumerinae (Cucurbitaceae) in the four northeastern provinces of Thailand was examined by using RAPD technique. Twenty 10-mer primers were produced 212 RAPD fragments, ranging from approximately 120 to 2531 bp. The genetic similarities were estimated from banding profiles using a NTSYS* version 2.1 as a basis for dendrogram construction via the UPGMA method. Cluster analysis divided the taxa under study into 2 clades. Moreover, a RAPD marker: Cm (OPJ11700) was specified to C. melo, and this marker was converted into sequence characterized amplified region (SCAR) marker: Cm (SCJ11516). A pair of sequence-specific primer of clones Cm (OPJ11700) amplified a distinct single band of the same size as the RAPD clones. The SCAR marker was developed successfully to identify C. melo genotype.
Highlights
Cucurbitaceae family contains about 90 genera and over 700 species of economic importance 1
DNA polymorphism in the cultivar species; Cucumis sativus L., C. melo L. and Benincasa hispida Cogn. of subtribe Cucumerinae (Cucurbitaceae) in the four northeastern provinces of Thailand was examined by using Randomly Amplified Polymorphic DNA (RAPD) technique
The amplicon, which was monomorphic to all the C. melo specimens but absent in other species was identified
Summary
Cucurbitaceae family contains about 90 genera and over 700 species of economic importance 1. PCR based methods including Randomly Amplified Polymorphic DNA (RAPD) 7 can be effectively used for the study of phylogeny and genetic diversity. RAPD markers have been used for the identification of cultivars and for assessing genetic diversity among cultivars of several crops like mungbean 8 , Lotus glaber Mill. Markers like RAPD based Sequence Characterized Amplified Region (SCAR) can increase industrial application of the molecular techniques. These markers have been used for authentication of plant species of Aquilaria (Thymelaeaceae) , Momordica charantia L. We use RAPD method to detect genetic variation and specific fragments among sample species, and converse the RAPD fragment into SCAR marker. It is necessary to provide an efficient and scientific method for assessing genetic diversity and species identification
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