Genetic diversity and relationship of pummelo germplasms in Hunan province based on morphological traits and SRAP molecular markers

Abstract

Germplasm resources is important material basis for plant genetic improvement,study on the genetic relationship and genetic diversity of pummelo germplasms are important for pummelo genetic improvement and new variety selection.In this paper,the genetic relationship and genetic diversity were studied based on morphological traits and the sequence-related amplified polymorphism(SRAP) molecular markers.The main results were as follows: ① The results of cluster analysis based on morphological traits using SPSS 17.0 software showed that the pummelo germplasms tested in the experiment could be divided into 6 groups when the index of genetic similarity was 10.② The UPGMA result showed that the genetic similarity index among the 47 pummelo germplasms varied from 0.65 to 0.93,which could be divided into 7 groups when the genetic similarity index was 0.798.③ 319 SRAP bands were acquired by PCR and 275 out of them were polymorphic,it was approximately 16.2 polymorphic bands per SRAP primers.The diversity ratio and the gene diversity value was 86.52% and 0.724 3 respectively.④ Compared the results of cluster analysis based on morphological traits and SRAP remarker,one could find that the 47 pummelo germplasm could be easily classified into different groups either by morphological traits or by SRAP remarker,and most of the pummelo germplasms could be separated from each other by the acquired special polymorphic bands of SRAP.