Genetic Diversity and Relationship among Bali Cattle from Several Locations in Indonesia Based on ETH10 Microsatellite Marker

Endang Tri Margawati,Indriawati Indriawati,Muhamad Ridwan,Slamet Diah Volkandari

doi:10.14334/jitv.v23i4.1915

Endang Tri Margawati, Indriawati Indriawati + Show 2 more

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https://doi.org/10.14334/jitv.v23i4.1915

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Abstract

<p>Bali cattle is one of local beef cattle in Indonesia, up to present its performance indicated an inbreeding occurrence. This study was aimed to analyze the genetic diversity and relationship among Bali cattle from several locations in Indonesia based on ETH10 microsatellite marker. Ninety-four (94) DNA samples (89 Bali cattle; 5 Banteng) were analyzed. The Bali cattle samples were from 6 locations in Indonesia (15 Pulukan; 15 Nusa Penida; 14 Bima West Nusa Tenggara/WNT; 10 Mataram, WNT; 20 Riau; 15 South Borneo). DNA Banteng samples were collected from Prigen Malang of East Java. Microsatellite marker of ETH10 labelled HEX was used for amplification. Alleles were analyzed by using Cervus 3.0.7 and GenAlex 6.5. Result showed that there were five (5) alleles found in ETH10 marker <em>i.e.,</em> 209; 213; 215; 217; and 219 bp. Average of observed (Ho) and expected (He) heterozygosity were 0.46±0.05 and 0.60±0.03, respectively. Five (5) out of 6 locations were in breeding occurrence except Bali cattle from Mataram was not inbreeding. The longest genetic relationship was between Bali cattle from Mataram and Riau whereas the closest distance was Bali cattle from South Borneo with Mataram. Banteng was closest to Bali cattle from Nusa Penida and the longest was to Bali cattle from South Borneo. This finding indicates there is inbreeding in Bali cattle, therefore it needs to be concerned in bull rotation and semen distribution for increasing the Bali cattle performance.</p>

Highlights

Bali cattle is one of beef cattle gene pools in South East Asia and as one of local beef cattle in Indonesia (Margawati et al 2015a)
Abnormal pattern of body color occurred in several locations of Indonesia i.e., Kupang, Nusa Tenggara Timur (NTT) (Tabun et al 2013), Lombok, Nusa Tenggara Barat (NTB) (Sudrana et al 2014), South Borneo (Lindell 2013), and Taro Gianyar, Bali island (Margawati et al 2015b)
The genetic diversity and relasionship was detected in the study of on ETH10 microsatelitte marker with five-variant alleles

Summary

Introduction

Bali cattle is one of beef cattle gene pools in South East Asia and as one of local beef cattle in Indonesia (Margawati et al 2015a). Bali cattle has been spread almost throughout the archipelago. Decreasing of performance (quantitative trait) and abnormal body color pattern is a sign of inbreeding occurrence. Abnormal pattern of body color occurred in several locations of Indonesia i.e., Kupang, Nusa Tenggara Timur (NTT) (Tabun et al 2013), Lombok, Nusa Tenggara Barat (NTB) (Sudrana et al 2014), South Borneo (Lindell 2013), and Taro Gianyar, Bali island (Margawati et al 2015b). The signs of abnormal color in Bali cattle are white spotted color, black color for cow, reddish body color for bull, reddish for soak leg color, and albino (Hardjosubroto & Astuti 1993).

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