Abstract
Sequence-related amplified polymorphism (SRAP) markers were used to investigate the genetic diversity among 30 populations of Toona ciliata Roem. sampled from the species’ distribution area in China. To analyze the polymorphism in the SRAP profiles, 1505 primer pairs were screened and 24 selected. A total of 656 SRAP bands ranging from 100 to 1500 bp were acquired, of these 505 bands (77%) were polymorphic. The polymorphism information content (PIC) values ranged from 0.32 to 0.45, with an average of 0.41. An analysis of molecular variance (AMOVA) indicated that the most significant variation was attributable to differences among the populations and that variation within the populations was small. STRUCTURE analysis divided the 30 populations into two parts. The unweighted pair group method of arithmetic averages (UPGMA) clustering and principal coordinates analysis (PCoA) showed that the 30 populations could be classified into four types. The results demonstrate a clear geographical trend for T. ciliata in China and provide a theoretical basis for future breeding and conservation strategy of T. ciliata.
Highlights
Toona ciliata Roem. is a deciduous or semi-deciduous tree
To the best of our knowledge, this study was the first time to use the Sequence-related amplified polymorphism (SRAP) molecular marker to evaluate the genetic diversity of T. ciliata
Results show that the novel SRAP marker was suitable for distinguishing T. ciliata
Summary
Toona ciliata Roem. (family Meliaceae) is a deciduous or semi-deciduous tree. It is one of the precious timber species in China and listed as a level II national key protected wild plant in the China Plant Red DataBook [1]. (family Meliaceae) is a deciduous or semi-deciduous tree. It is one of the precious timber species in China and listed as a level II national key protected wild plant in the China Plant Red Data. The flowers are small and hermaphroditic, with the pollen spread primarily by wind. The seeds are light with wings, dispersed mainly by wind . Low natural regeneration and over-exploitation have resulted in the continual decline of T. ciliata leading to it being classified as an endangered species.
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