Abstract
Genetic diversity and population structure of the peanut worm (Sipunculus nudus) were investi- gated by using 536 base-pair fragments of the mitochondrial 16S ribosomal gene. Populations were collected from three locations along the southern coast of China - Beihai, Sanya, and Xiamen. Amplified polymerase chain reaction products were sequenced in both directions and data were analyzed using ClustalX, Arlequin, and MEGA. A total of 69 polymorphic sites and 21 distinct haplotypes were revealed. The mean haplotype and nucleotide diversity of the three pop- ulations were 0.814% and 0.37%, respectively. The Beihai population had the greatest haplo- type and nucleotide diversity, followed by the Xiamen and Sanya populations. Analysis of mole- cular variance (AMOVA) showed significant genetic differentiation among the three populations (Fst = 0.0619, p<0.05) and distinct population structures among the three sites.
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