Genetic Diversity and Population Structure of Coilia mystus in Yangtze Estuary and Its Adjacent Waters Based on Mitochondrial COI Gene

Abstract

Background: Coilia mystus is an important commercial and ecologically species. In recent years, its resources have declined sharply. It is very important to establish management unit for better protect this species. At present, the population genetic structure of C. mystus from the adjacent waters has not been studied. The current study aimed to study the population genetic structure of C. mystus from the adjacent waters, so as to establish the management unit for better protect and management its resources. Methods: Samples of 144 C. mystus individuals were collected from 4 localities of LS, CM, ZS and WZ for total genomic DNA extraction. PCR amplification was carried out by the common pairs of primers and the target amplification products were separated, purified and sequenced. The COI sequences and genetic diversity were analyzed by a series of sequence analysis software. Result: The results identified low genetic diversity and significant genetic differentiation of C. mystus from Yangtze Estuary and its adjacent waters. The influence factors of genetic diversity including historical factors, anthropogenic activity, habitation and a low rate of gene flow were discussed. The present work will conducive to the establishment of management unit for better protection and utilization of this species.