Genetic Diversity and Population Structure Analysis of Landrace and Wild Relatives of Lentil Germplasm Using CBDP Marker

Abstract

Assessment of genetic diversity and population structure of germplasm collections provides an opportunity for plant breeders to develop new and improved cultivars. In this study, genotypic variation of 90 lentil genotypes was evaluated using 10 CAAT Box Derived Polymorphism (CBDP) markers. The polymorphism percentage was 100% with an average of 100% indicating a high polymorphism level. A total of 117 alleles were identified for different genetic locations and with an average of 13 alleles per primer. Among CBDP markers used, the highest allele amplified belonged to CBDP22 marker with 21 bands and the lowest allele amplified corresponded to CBDP3, CBDP6 and CBDP13 markers with 11 bands. The Polymorphic information content (PIC) ranged from 0.413 to 0.471 with an average of 0.448. Marker Index (MI) ranged from 4.85 to 9.90. The highest index for genetic diversity (PIC and MI) belonged to the CBDP22 indicating a high resolution for this marker as compared to other markers. Based on neighbor joining clustering for molecular data, genotypes were grouped into five distinct groups. Population structure analysis showed that the highest peak was at K = 3, indicating the presence of three major clusters. The results of this study showed that CBDP markers can be used as a useful tool for studying the genetic diversity of lentil germplasm.