Abstract
White clover is an important temperate legume forage with high nutrition. In the present study, 448 worldwide accessions were evaluated for the genetic variation and polymorphisms using 22 simple sequence repeat (SSR) markers. All the markers were highly informative, a total of 341 scored bands were amplified, out of which 337 (98.83%) were polymorphic. The PIC values ranged from 0.89 to 0.97 with an average of 0.95. For the AMOVA analysis, 98% of the variance was due to differences within the population and the remaining 2% was due to differences among populations. The white clover accessions were divided into different groups or subgroups based on PCoA, UPGMA, and STRUCTURE analyses. The existence of genetic differentiation between the originally natural and introduced areas according to the PCoA analysis of the global white clover accessions. There was a weak correlation between genetic relationships and geographic distribution according to UPGMA and STRUCTURE analyses. The results of the present study will provide the foundation for future breeding programs, genetic improvement, core germplasm collection establishment for white clover.
Highlights
White clover (Trifolium repens L.) is a cool-season, allotetraploid (2n=4x=32) perennial legume species (Cogan et al, 2006; Isobe et al, 2012)
The optimal K capturing the major structure in the white clover data was determined using Structure Harvester (Earl & vonHoldt, 2012; Evanno, Regnaut & Goudet, 2005)
The percentage of polymorphic bands revealed different levels of polymorphisms ranging from 91.67% to 100%
Summary
White clover (Trifolium repens L.) is a cool-season, allotetraploid (2n=4x=32) perennial legume species (Cogan et al, 2006; Isobe et al, 2012) It can grow well in a wide range of soil and environmental conditions with proper management, and it has extended its range globally by wild and cultivated distribution from its natural range (Europe, Western Asia, and North Africa) (Griffiths et al, 2019). 22 microsatellite markers (Griffiths et al, 2013; Griffiths et al, 2019) were used to evaluate the genetic variation among 448 white clover accessions collected from globally diverse origins. Our results have important implications for future breeding, germplasm improvement, and core germplasm collection in white clover
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
