Abstract
Seventeen haplotypes were detected from the complete mitochondrial DNA control region sequences analyzed from eighty individuals of two Tibetan domestic sheep breeds. The nucleotide composition of all the sequences was 33.0% A, 29.7%T, 22.9%C and 14.4%G; G+C was 37.3%. The length of the sequences ranged from 1,107 bp to 1,259 bp. The difference between them was primarily due to 3-5 copy numbers of a 75 bp tandem repeat sequence. The NJ phylogenetic tree (the number of replications of bootstrap test is 1,000) presented three major domestic sheep lineages, which suggested that modern Tibetan sheep breeds are derived from three maternal sources.
Highlights
Seventeen haplotypes were detected from the complete mitochondrial DNA control region sequences analyzed from eighty individuals of two Tibetan domestic sheep breeds
The length variations were caused by different copy numbers of a 75 bp tandem repeat except for minor insertions and deletions
The copy numbers of the tandem repeat were present randomly in the haplotypes, so they could not be seen as being characteristic of the haplotypes
Summary
Seventeen haplotypes were detected from the complete mitochondrial DNA control region sequences analyzed from eighty individuals of two Tibetan domestic sheep breeds. The results are useful for the conservation and utilization of Chinese sheep genetic resources. The mtDNA D-loop, we analyzed the genetic diversity of The PCR amplification reaction system consisted of two breeds and inferred their phylogenetic status. Haplotype analysis in Chinese Tibetan sheep The length of the sequences varied considerably between 1,107 bp and 1,259 bp, but most of the sequences were 1,181 bp.
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