Genetic Diversity and Phylogenetic Analyses of Laurus nobilis L. (Lauraceae) Populations Revealed Chloroplast (cpDNA) trnL Intron and trnL-F Region

Abstract

ABSTRACT In this study, genetic diversity and phylogenetic analyzis based on cpDNA trnL intron and trnL-F sequences of L. nobilis L. populations were made. Persea schiedeana and Endiandra wolfei taxa were used as outgroups. Plant samples were collected from various different provinces and districts of the Aegean region and brought to the laboratory. Genomic DNAs were isolated from fresh and green leaves of the plants. trnC and trnD primers were used for the trnL intron region, and trnE and trnF primers were used for the trnL-F region. The obtained DNA sequences were then corrected using BioEdit and FinchTV programs. MEGA 6.0 program was used both phylogenetic analyzis and the detection of genetic distances. For the intron region of the trnL, the mean nucleotide composition was determined as 36.5% T, 20.2% C, 26.4% A and 16.9% G. These values were found as 30.7% T for 15.9% C, 31.9% A and 21.6% G for the trnL-F region. The lowest distance for the trnL intron region was found 0.000 while the highest distance was 0.004. The value was found 0.000 for the trnL-F region. The phylogenetic tree, which was obtained using both trnL intron and trnL-F sequences, consisted of two large branches. As a result, according to the data obtained from trnL intron and trnL-F sequences, the trnL intron sequence found a solvent in the phylogenetic relationship between L. nobilis populations, while the phylogenetic relationship between trnL-F sequence and L. nobilis populations was not found.