Abstract
The present investigation has been undertaken with the objective to study the genetic variability and diversity among the Naga king chilli genotypes using SSR marker at Medziphema during kharif season of 2018. All total 89 bands were produced by 30 primers, 37 bands being polymorphic and producing average percentage polymorphic bands of 41.66 %. The Primer CAMS-826 showed highest percentage of polymorphic bands (75%), followed by primer CAMS 032, CAMS-117, CAMS- 864 and CAMS-647 which revealed (66.66 %) and primer CAeMS 009, CAMS 163, CAMS 871, HmpsE 047 AND HpmsE 014 exhibited 50% polymorphism which indicates that the primer can be used for genetic diversity study of C. chinense genotypes. Genetic parameters analyzed using Popgene® software indicated that Peren population has higher diversity among the four region populations. Jaccard’s similarity coefficient between the genotypes ranged from 0.64 to 0.96, indicating moderate variation between the genotypes. Clustering analysis depicted three clusters where germplasm collected from Peren and Medziphema showed close relatedness and Principal Coordinate Analysis (PCo A) plot obtained also supports cluster analysis data. Germplasm evaluation is of immense important for genetic crop improvement.
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