Abstract
The purpose of the present study was to investigate the genetic diversity and molecular characterization of 50 soybean genotypes in Bangladesh with 20 SSR markers. Genomic DNA was isolated by modified standard cetyl tri-methyl ammonium bromide (CTAB) extraction protocol and alleles were amplified by polymerase chain reaction (PCR). Allele sizes were estimated in comparison with 50 bp DNA ladder. The software NTSYSpc version 2.2 and POPGENE version 1.31 were utilized for molecular data analysis and preparation of dendrogram. Polymorphic Information Content (PIC) values varied from 0.53 (Satt664) to 0.98 (Satt009, Satt330 and Satt522) with the mean value 0.897 and expected heterozygosity varied from 0.4059 (Satt685) to 0.1246 (Satt664) with the mean value 0.244. The dendrogram analysis depicted that the 50 genotypes were grouped in four (4) major clusters. The most diverse genotypes were SBG-1, PM-78-6-3-13, BS-3 and AGS-31, which suggest that the simple sequence repeat (SSR) markers are very efficient for genetic diversity analysis. The similarity matrix revealed the diversity among genotypes. The diverse genetic materials obtained from the present study on genetic diversity of soybean genotypes in Bangladesh may be utilized in the future breeding programme.
Highlights
Soybean (Glycine max (L.) Merr.) is the most vital economic legume crops in the world
The genetic variability or similarity analysis is very important for the protection of soybean germplasm, their improvement and efficient management
The polymerase chain reaction (PCR) based fingerprinting assay, i.e., simple sequence repeat (SSR) or microsatellite markers were used for the evaluation of 50 soybean genotypes
Summary
Soybean (Glycine max (L.) Merr.) is the most vital economic legume crops in the world. It supplies vegetable oil which is a crucial source of protein for human and animal nutrition. The genetic diversity analysis is important to broaden the genetic base of soybean germplasm which can be further used in breeding programs. Molecular markers were used for the identification of genetically diverse genotypes which were further used in a breeding program (Maughan et al, 1996; Thompson & Nelson, 1998). Molecular markers were used to evaluate genetic variation within the collected germplasms of soybean genotypes which was reported by Mohammadi and Prasanna (2003)
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