Abstract
The molecular marker is a useful tool for assessing genetic variations and resolving cultivar identities. Information on genetic diversity and relationships among rice landraces from Bangladesh is currently very limited. Thirty-five rice genotypes including 33 landraces and 01 HYV of Bangladesh and 1 Indian landrace of particular interest to breeding programs were evaluated by means of random amplified polymorphic DNA (RAPD) technique. For molecular characterization, RAPD markers viz., OPC 03, OPC 04 and OPA 01 gave reproducible and distinct polymorphic amplified products. A total of 20 RAPD bands were scored of which 15 polymorphic amplification products were obtained by using these arbitrary primers. The size of amplified fragments were ranged from 550 to 1775 bp. Based on analysis performed on a similarity matrix using UPGMA, 35 genotypes were grouped into 2 main clusters. Landrace Sylhet balam and Mota aman was totally different from other genotypes. The information will facilitate selection of genotypes to serve as parents for effective rice breeding programs in Bangladesh. DOI: http://dx.doi.org/10.3329/ijarit.v4i1.21099 Int. J. Agril. Res. Innov. & Tech. 4 (1): 77-87, June, 2014
Highlights
Rice (Oryza sativa L.) diversity consists of landraces, improved cultivars, hybrids and closely related wild relatives
To formulate a sustainable breeding program precise knowledge about genetic divergence for yield components is a crucial one as varietal improvement depends mainly on the selection of parents with high genetic divergence in hybridization that is supposed to increase the chance of obtaining maximum heterosis and give broad spectrum of variability in segregating generations
polymerase chain reaction (PCR)-based molecular marker technique random amplified polymorphic DNA (RAPD) analysis is a reliable tool for assessing genetic diversity
Summary
Rice (Oryza sativa L.) diversity consists of landraces, improved cultivars, hybrids and closely related wild relatives. PCR-based molecular marker technique RAPD analysis is a reliable tool for assessing genetic diversity. This technique use a single short oligonucleotide primer (9-10 bp) of arbitrary DNA sequence and polymerase chain reaction (PCR) mediated amplification of random fragment from genomic DNA. Advantages associated with RAPDs have made them a favorite marker technique in mapping, the determination of phylogenetic relationships, genetic diversity, and identification of cultivars and parents in a number of plant species. Since primers can be chosen arbitrarily, any organism can be mapped with the same set of primers These advantages make RAPD markers far easier to work. The objective of this present study was to evaluate genetic divergence of 35 rice genotypes with three RAPD markers
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