Genetic Diversity Analysis of Hybrid Rice Parental Lines and Genetic Purity Assessment of Hybrid Seeds of China

Haiya Cai,Yuxia Lu,Shuo Zhang,Aiqing You,Gang Liu,Haitao Jia,Chunhai Jiao

doi:10.5539/jas.v12n5p37

Abstract

Thirty-five pairs of SSR primers were used for genetic diversity analysis and DNA fingerprinting of 31 hybrid rice core parental lines developed in central- and southern-China using one japonica rice line and three inbred rice lines as the check varieties. The average number of alleles (Na) per SSR locus was 4.02, with a range of two to eight, the effective number of alleles (Ne) was 83.16 with a mean of 2.38, ranging from 1.19 to 4.66. The polymorphic information content (PIC) ranged from 0.16 to 0.79, with an average number of 0.52. The results of the cluster analysis indicated that the check varieties viz., one japonica rice and three inbred rice, were clustered into two groups with similarity coefficients of 0.62 and 0.71 respectively indicating their relatedness. Thirty-one hybrid rice parental lines were clustered into 6 groups according to their different types, pedigrees and regions of development with similarity coefficients of approximately 0.76. The highest genetic similarity coefficient (0.94) was observed between Y58S and C815S, and the lowest (0.63) was observed between Quan9311A and Peiai64S. The purity of one hybrid rice cultivar was tested using characteristic marker and the field test, and it was demonstrated that the purities obtained using the two methods were similar. This research will be helpful for rice breeding, new cultivar registration and seed production.

Highlights

IntroductionRice is the consumed staple food by more than half of the people in the world (Zhang et al, 2007)
Rice is the consumed staple food by more than half of the people in the world (Zhang et al, 2007).Approximately 70 percent in China is dependent on rice, that necessitated more attention to its varietal improvement
This research will be helpful for rice breeding, new cultivar registration and seed production

Summary

Introduction

Rice is the consumed staple food by more than half of the people in the world (Zhang et al, 2007). 70 percent in China is dependent on rice, that necessitated more attention to its varietal improvement. The development of new rice varieties in China has been continuously increased in recent years. 565 varieties were developed in 2008, and 943 varieties were developed in 2018, among which indica rice accounted for 77 percent, and hybrid rice accounted for 72 percent (http://www.ricedata.cn/variety/index.htm). Due to rapid increase in quantity of developed hybrids, a better method for variety identification and genetic diversity analysis is required. Four basic requirements should be met in crop variety identification: stability of the environment, ability to identify existing variation among varieties, minimum variation within varieties and reliability of the results.

Methods

Results

Discussion

Conclusion