Genetic diversity analysis of banana using SSR markers

Abstract

Banana, a crucial fruit crop thriving in tropical and sub-tropical regions, facing a notable challenge of elevated sterility across various cultivated banana genotypes. This sterility issue hampers the cultivation of diverse banana varieties. Therefore, a focused endeavour is crucial to thoroughly investigate the richness of diversity within both wild and cultivated banana genotypes, aiming to preserve it for future utilization. In the present study, 21 Musa genotypes were collected from different places of North-Eastern Region, and conserved in the field gene bank at Department of Horticulture, College of Agriculture, Lembucherra, Tripura during 2015-2022. Molecular characterization and DNA fingerprinting of these banana accessions were performed by employing 16 banana SSR markers. The number of alleles per locus ranged from 15 to 24 with an average 20.375. The expected heterozygosity (He) ranged from 0.564-0.967 with a mean of 0.852, and the polymorphic information content (PIC) varies from 0.638–0.945 (mean = 0.859). Genetic diversity analysis showed dissimilar groups, indicating possibility of involvement of different accuminata and balbisiana types in evaluation of banana in NE region. The outcome of the current study will be useful for des igning banana crops against different stresses