Genetic Diversity Analysis of Aydın/Turkey Dittrichia viscosa (L). Greuter (Asteraceae) Populations Using RAPD Markers

Abstract

In this study, we performed a genetic diversity using RAPD markers for some Dittrichia viscosa populations grown in the Aydın region of Turkey. Total genomic DNA isolation from the leaves of Dittrichia viscosa was performed using commercial kit. eight RAPD primers were used to determine genetic diversity among populations. Polymerase Chain Reaction (PCR) was performed with all DNA samples and primers with ability of scoreing band. PCR products were run in agarose gel and visualized under UV light. They scored as there were bands (1) and no bands (0) at all gel images and their files were created. A total of 50 characters were obtained from the primers. Phylogenetic relationships and genetic distances between the cultivars were calculated by using the PAUP* 4.0b10 analysis program. According to the PAUP data, the closest genetic distance was determined 0.20000 and between Central and İncirliova populations, the most distant genetic distance were determined 0.36842 between İncirliova and Koçarlı populations. In the phylogenetic analysis has been obtained using UPGMA algorithms and phylogenetic tree consist of two clades. The results also propose that RAPD markers are useful tools for indicating genetic relationships among Dittrichia viscosa populations.