Genetic Diversity Analysis among Fennel Genotypes Employing Gene Targeted Molecular Markers, CAAT-Box Derived Polymorphism (CBDP) and Conserved DNA Derived Polymorphism (CDDP) Markers

Abstract

Genetic diversity analysis was carried out in 14 varieties of Foeniculum vulgare by using CAAT-box derived polymorphism (CBDP) and conserved DNA derived polymorphism (CDDP) markers. DNA was isolated from young leaves followed by a standard CTAB method. A total of 25 CBDP primers were screened to study the diversity and with all 25 primers were amplifying reproducible bands, and 21 CDDP primers were screened, out of which 20 primers could show amplification. A part of that CDDP 17 primer could not amplify reproducible bands. Which produced a total of 222 bands (8.0 bands per primer), of which 151 were polymorphic. The analysis revealed 66.14% polymorphism for CBDP.CDDP analysis produced a total of 258 bands (12.0 bands per primer), of which 148 were polymorphic. The analysis revealed 56.18% polymorphism. The polymorphism was the maximum (100%) with primer CBDP 24 and the lowest (12.5%) with primer CBDP-11. Similarly, the polymorphism was the maximum (92.30%) with primer CDDP 08 and the lowest (0%) with primers CDDP 10 and CDDP 13. All the CBDP and CDDP primers generated polymorphic markers in fennel cultivars, and an UPGMA dendrogram based on Jaccard's similarity grouped. Interestingly, they could potentially be exploited in other species as well for assessing genetic diversity, cultivar identification, construction of linkage map, and markerassisted selection.