Abstract

Silver Genetic diversity of Colletotrichum falcatum causing red rot of sugarcane was assessed based on morphological, pathological and molecular characteristics especially from sub-tropical Indian conditions. Sixteen isolates of this pathogen were collected based on the extensive survey on prominent varieties grown in the region along with some elite selections. Morphological observations (colony colour, mycelium pattern and sporulation) grouped the isolates into two distinct types (C1: light type and C2: dark type). However, quantitative data on colony diameter showed five clusters for these isolates. Pathogenic characterization of these isolates on fourteen standard differentials formed six groups, ingroup 1: (CF-Pb-1) isolates Cf-157, Cf-249 and Cf-248 were the most virulent while group 6 (CF-Pb-6) isolates Cf-60 and Cf-247 were the least one. The genetic relatedness among the isolates using Random Amplified Polymorphic DNA (RAPD) analysis revealed sufficient molecular polymorphism, which in turn confirmed the variation in virulence of different isolates. The data categorized different isolates into two major clusters and five independent lineages. Polymorphic information content (PIC) ranged from 0.701 to 0.929. Isolate Cf-223 was found to be genetically most diverse among all the isolates. Present study inferred that morphological grouping of most of the isolates showed positive correlation with the pathogenic variability while molecular diversity did not showed such associations.

Highlights

  • Sugarcane (Saccharum spp.) is one of the most important nature of pathogen in the form of different strains, which agro-industrial crops of India next to textile industry

  • Sixteen isolates of C. falcatum were isolated from red rot infected stalks of 15 sub-tropical sugarcane cultivars from different sugar mill areas of Punjab, Haryana and Uttar Pradesh (Tab. 1)

  • Results and discussion in group V exhibited slow colony growth with diameter of 73.0 mm (Tab. 2). Such morphological and cultural variation in C. falcatum had been studied by earlier workers in different sugarcane varieties (Prakasam and Venkatareddy, 1961; Sharma, 1970)

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Summary

Introduction

Sugarcane (Saccharum spp.) is one of the most important nature of pathogen in the form of different strains, which agro-industrial crops of India next to textile industry. Present study was undertaken to apply different approaches morphological, pathological and molecular to investigate the existing variability of C. falcatum isolates in northern India. Pathogenicity assay Fourteen sugarcane differentials namely Baragua (S. officinarum), Khakai (S. sinense), SES 594 (S. spontaneum), CoS 767, Co 975, BO 91, CoC 671, Co 7717, Co 997, CoJ 64, Co 1148, Co 419, Co 62399 and Co 8436 were used to study pathogenic variability of C. falcatum under field conditions.

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