Abstract
We report total ten isolates of F. semitectum recovered from different hosts. Identity of these isolates was determined by morphological and cultural characteristics and confirmed by RAPD-PCR analysis using forty random primers. Morphologically all the ten isolates showed similarity, but based on RAPD-PCR analysis, these isolates can be categorized in three groups depending upon similarity co-efficient. Genetic similarity coefficients between pair wise isolates varied from 0.00 to 1.95 based on an unweighted paired group method of arithmetic average (UPGMA) cluster analysis. RAPD-PCR technique can be used as an important tool for the genetic differentiation among isolates of F. semitectum.
Highlights
Fusarium is one of the most important genus of plant pathogenic fungi [1,2]
Ten isolates of F. semitectum were recovered from different infected hosts (Table 1) on Potato dextrose agar (PDA) and cultured in pure form from a single spore culture
The morphological characters of different isolates of F. semitectum species in the present study showed the resemblance with morphological characters reported by many researchers in past [12,19,20,21]
Summary
Fusarium is one of the most important genus of plant pathogenic fungi [1,2]. It causes infection in plants, animals and human beings [3,4,5,6]. In India, chilli (Capsicum annuum L.) suffered with a characteris- tic leaf curl symptoms due to the attack of mite, (Polyphagotarsonemus latus) (Banks) and thrips (Scirtothrips dorsalis) or both In such cases F. semitectum was found to be active against these mites and thrips. Different keys are available for the identification of Fusarium species on the basis of morphological and cultural characteristics. RAPD has the advantage of being technically simple and rapidly facilitated It includes use of random primers and there is no need to have genetic information of test organism. Fragariae causing wilt disease in strawberry was studied using different molecular markers including RAPD [18]. Ten isolates of F. semitectum isolated from different infected plant materials including fruits, vegetables and foodgrains were identified on the basis of morphological and cultural characteristics. The intra-species genetic variations among these isolates were studied by using RAPD-PCR method
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
