Abstract
Abstract The objectives of this study were evaluation of genetic diversity and marker–trait association of 64 barley ( Hordeum vulgare L.) genotypes using hordeins and simple sequence repeats (SSRs) markers under optimal moisture and drought stress conditions. Moreover, to evaluate the response of barley genotypes to drought stress, five drought tolerance indices were calculated. SSRs and hordeins generated clear patterns with high polymorphism. SSRs and hordeins analysis provided us with useful information on the level of polymorphism and diversity in barley. Marker–trait associations were studied for 22 agronomic traits using 122 SSR markers (obtained from 14 primer pairs) and 51 hordeins bands in 64 barley genotypes under both normal and stress conditions. Phenotypic traits strongly associated with SSRs were also strongly associated with hordeins. Generally, we believed that at least some of these markers would be informative and validated and can be used in marker-assisted selection (MAS) under drought stress.
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