Genetic divergence in popcorn lines detected by microsatellite markers

T.S Dandolini,C.A Scapim,M.F.P.S Machado,C.A Mangolin,A.D Lopes,A.S Mott,A.T Amaral Júnior

doi:10.12702/1984-7033.v08n04a09

Abstract

The purpose of this study was to use microsatellite markers to evaluate the genetic divergence in 10 popcorn lines. The number of alleles per locus ranged from 2 to 5. The proportion of polymorphic loci was highest (50%) in line Curagua, while Zelia II was the most monomorphic (100%). The arithmetic complement of similarity of Roger and Tanimoto for the 10 lines indicated lower genetic similarity between the lines Zelia II and Avati Pichinga I and higher similarity between Zelia I and Zelia II. The mean distance between groups was greatest between the group formed by lines IAC 112 I 112 IAC II, Avati Pichinga I, Avati Pichinga II and the group containing Yellow Pear Popcorn II. Strategies on how to use the heterosis between different lines in partial diallel crosses are indicated.

Highlights

The most recent trend in breeding programs is to integrate traditional techniques and biotechnology
For the hybrid breeding programs the potential of DNA markers provides the identification of divergent genotypes which exploit heterosis for the yield components in populations and improved hybrids (Benchimol et al 2000, Warburton et al 2002, Aguiar et al 2008, Vilela et al 2008)
Molecular markers are advantageous since they discriminate little different lines and, heterotic groups are composed containing genotypes that unequivocally represent the differences in allele frequencies of the populations

Summary

Introduction

The most recent trend in breeding programs is to integrate traditional techniques and biotechnology. For the hybrid breeding programs the potential of DNA markers provides the identification of divergent genotypes which exploit heterosis for the yield components in populations and improved hybrids (Benchimol et al 2000, Warburton et al 2002, Aguiar et al 2008, Vilela et al 2008). Molecular markers are advantageous since they discriminate little different lines and, heterotic groups are composed containing genotypes that unequivocally represent the differences in allele frequencies of the populations. These studies are consistent, especially since the highly efficient biometric information for the identification of promising lines in crosses is not discarded, such as estimates of combining ability or knowledge on the germplasm pedigree. While yield depends in the first place on dominance effects, the expansion volume - main trait of grain quality - is influenced almost exclusively by additive effects Lyerly (1942), Pereira and Amaral Júnior (2001), Scapim et al (2002), Simon et al (2004), Freitas Júnior et al (2006), Rangel et al (2007), Rangel et al (2008), Santos et al (2008)

Objectives

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Results