Abstract
Random Amplified Polymorphic DNA (RAPD) assay was conducted to identify polymorphic markers in Amrithmahal, Krishna Valley, Hallikar, Deoni, Khillari, Ongole and Malnad Gidda breeds of South Indian cattle using twenty six primers. Of the 93 RAPD markers obtained, 53 were present in all breeds, 22 were individual specific and 18 were polymorphic for different breeds. Dual purpose breeds viz., Krishna Valley and Ongole showed less genetic divergence between them as compared to their genetic divergence from draft breeds viz., Amrithmahal, Hallikar and Khillari. Malnad Gidda was found to be a distinctly different from others studied. (Asian-Aust. J. Anim. Sci. 2002. Vol 15, No. 3 : 309-314)
Highlights
Zebu breeds of cattle from Southern India are well known for their draft efficiency and resistance to diseases
PCR primers Based on earlier reports on Random Amplified Polymorphic DNA (RAPD) studies in zebu breeds, 26 arbitrary oligonucleotide primers obtained from Bangalore Genei (P) Ltd., Bangalore, India were used for amplification of the breed specific DNA pools
It is well established that DNA fingerprints can be successfully used to compare genetic distances between human beings (Gill et al, 1985), sheep (Cushwa et al, 1996, Dodgson et al, 1996), dogs and cats (Jefferys and Morton, 1987) and Bos indicus and Bos taurus cattle (Kemp and Teale, 1994)
Summary
Zebu breeds of cattle from Southern India are well known for their draft efficiency and resistance to diseases. India (Amrithmahal, Krishna Valley, Hallikar, Deoni, Khillari, Ongole and Malnad Gidda) with their home tract (figure 1), were sampled from different herds. Blood samples from 150 animals of Amrithmahal, 150 of Krishna Valley, 45 of Deoni, 39 of Hallikar, 19 of Khillari, 9 of Ongole and 27 of Malnad Gidda were obtained.
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