Genetic dissection of the role of ethylene in regulating auxin-dependent lateral and adventitious root formation in tomato

Sangeeta Negi,Poornima Sukumar,Jerry D Cohen,Xing Liu,Gloria K Muday

doi:10.1111/j.1365-313x.2009.04027.x

Abstract

In this study we investigated the role of ethylene in the formation of lateral and adventitious roots in tomato (Solanum lycopersicum) using mutants isolated for altered ethylene signaling and fruit ripening. Mutations that block ethylene responses and delay ripening -Nr (Never ripe), gr (green ripe), nor (non ripening), and rin (ripening inhibitor) - have enhanced lateral root formation. In contrast, the epi (epinastic) mutant, which has elevated ethylene and constitutive ethylene signaling in some tissues, or treatment with the ethylene precursor 1-aminocyclopropane carboxylic acid (ACC), reduces lateral root formation. Treatment with ACC inhibits the initiation and elongation of lateral roots, except in the Nr genotype. Root basipetal and acropetal indole-3-acetic acid (IAA) transport increase with ACC treatments or in the epi mutant, while in the Nr mutant there is less auxin transport than in the wild type and transport is insensitive to ACC. In contrast, the process of adventitious root formation shows the opposite response to ethylene, with ACC treatment and the epi mutation increasing adventitious root formation and the Nr mutation reducing the number of adventitious roots. In hypocotyls, ACC treatment negatively regulated IAA transport while the Nr mutant showed increased IAA transport in hypocotyls. Ethylene significantly reduces free IAA content in roots, but only subtly changes free IAA content in tomato hypocotyls. These results indicate a negative role for ethylene in lateral root formation and a positive role in adventitious root formation with modulation of auxin transport as a central point of ethylene-auxin crosstalk.

Highlights

The development of lateral and adventitious roots is a highly plastic process which is sensitive to nutrients, moisture, and other environmental parameters, with plant hormones acting as one important signaling mechanism (Malamy and Ryan, 2001; Li et al, 2009)
We examined the effect of ethylene on free indole-3-acetic acid (IAA) levels in these plants and examined the effect of ethylene on auxin transport in the primary root and hypocotyl
We examined mutants defective in ethylene signaling and fruit ripening to ask if ethylene modulates root formation in tomato

Summary

Introduction

The development of lateral and adventitious roots is a highly plastic process which is sensitive to nutrients, moisture, and other environmental parameters, with plant hormones acting as one important signaling mechanism (Malamy and Ryan, 2001; Li et al, 2009). Quiescent cells within their pericycle layer begin dividing and form lateral root primordia via a precise series of divisions, which are best characterized in Arabidopsis (Malamy and Benfey, 1997). When shoot tissues of many plant species contact the soil, they can undergo an intriguing, but poorly characterized, process by which shoot tissues differentiate a 2009 The Authors Journal compilation a 2009 Blackwell Publishing Ltd to form adventitious roots. Plant propagation relies heavily on the ability of shoot cuttings to effectively generate adventitious roots, yet there is dramatic variation between species in their propensity to form adventitious roots (De Klerk et al, 1999) and little molecular information on this important developmental process

Results

Discussion

Conclusion