Genetic Differentiation of Pseudomonas syringae Pathovar tomato from Other P. syringae Pathovars using REP-PCR and URP-PCR

Abstract

For the genetic differentiation of <TEX>$Pseudomonas$</TEX> <TEX>$syringae$</TEX> pathovar <TEX>$tomato$</TEX>, a total of 51 <TEX>$P.$</TEX> <TEX>$syringae$</TEX> pv. strains infecting 33 different host plants were analyzed using repetitive element PCR(REP-PCR) and universal rice primer PCR(URP-PCR). The entire DNA fingerprint profiles were analyzed using unweighted pair-group method with arithmetic averages (UPGMA). The 51 <TEX>$P.$</TEX> <TEX>$syringae$</TEX> pv. strains could be divided into five clusters based on 65% similarity by Rep-PCR using BOX, ERIC, and REP primers. <TEX>$P.$</TEX> <TEX>$syringae$</TEX> pv. <TEX>$tomato$</TEX> cluster was well separated from other 31 <TEX>$P.$</TEX> <TEX>$syringae$</TEX> pathovars. <TEX>$P.$</TEX> <TEX>$syringae$</TEX> pv. <TEX>$tomato$</TEX> cluster included only <TEX>$P.$</TEX> <TEX>$syringae$</TEX> pv. <TEX>$maculicola$</TEX> and <TEX>$P.$</TEX> <TEX>$syringae$</TEX> pv. <TEX>$tomato$</TEX>. <TEX>$P.$</TEX> <TEX>$syringae$</TEX> pv. <TEX>$tomato$</TEX> strains could be divided into two genetic groups. Meanwhile, the Pseudomonas pv. strains could be divided into four clusters based on 63% similarity by URP-PCR using 2F, 9F, and 17R primers. <TEX>$P.$</TEX> <TEX>$syringae$</TEX> pv. <TEX>$tomato$</TEX> cluster was also well separated from 30 other <TEX>$P.$</TEX> <TEX>$syringae$</TEX> pathovars. In this case, <TEX>$P.$</TEX> <TEX>$syringae$</TEX> pv. <TEX>$tomato$</TEX> cluster included <TEX>$P.$</TEX> <TEX>$syringae$</TEX> pv. <TEX>$maculicola$</TEX>, <TEX>$P.$</TEX> <TEX>$syringae$</TEX> pv. <TEX>$berberidi$</TEX>, and <TEX>$P.$</TEX> <TEX>$syringae$</TEX> pv. <TEX>$tomato$</TEX>. <TEX>$P.$</TEX> <TEX>$syringae$</TEX> pv. <TEX>$tomato$</TEX> strains was also separated into two genetic groups by URP-PCR analysis. Overall, our work revealed that <TEX>$P.$</TEX> <TEX>$syringae$</TEX> pv. <TEX>$tomato$</TEX> can be genetically differentiated from other <TEX>$P.$</TEX> <TEX>$syringae$</TEX> pathovars by the DNA fingerprint profiles of REP-PCR and URP-PCR. We first report that there are two genetically diverged groups in <TEX>$P.$</TEX> <TEX>$syringae$</TEX> pv. <TEX>$tomato$</TEX> strains.