Abstract
Genetic diversity and differentiation of 58 C. truncatum isolates from urdbean, horsegram, soybean, ricebean, cowpea and mungbean collected from the traditional crop growing area of north-western Himalayas was studied through RAPD analysis. RAPD profiles generated by the random primers exhibited a high degree of variability among different isolates of C. truncatum. Cluster analysis of the binary data permitted the grouping of isolates on the basis of their origin from different hosts. However, isolates from cowpea and mungbean collected from the same field were grouped together. The dendrogram revealed the presence of 52 multilocus genotypes among 58 isolates of the pathogen. Maximum diversity was detected in the isolates from ricebean and horsegram (′D = 1) followed by urdbean (′D = 0.99). The results indicate that at least some gene flow is occurring between the different host-limited forms of C. truncatum.
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