Abstract
Genetic variation among 14 isolates of Phaeosphaeria nodarum and 10 isolates of P. avenaria that originated from diverse geographic locations was assessed by the use of restriction fragment length polymorphisms (RFLPs). Genomic DNAs were digested with the restriction enzyme EcoRI and hybridized with 38 anonymous DNA probes. Twenty of the 38 probes were isolated from P. nodorum, and the other 18 probes were isolated from P. avenaria. Most of the probes hybridized with one or two DNA bands per isolate. Each isolate was assigned a RFLP genotype, which is a combination of the banding patterns with all 38 probes. Isolates of P. nodorum showed a significantly lower degree of genetic variation than the isolates of P. avenaria [...]
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