Abstract
The importance of atmospheric nitrogen-fixing bacteria The study aimed to isolate bacteria from the roots of plants From four areas of the city of Mosul the winter agriculture, season 2020-2021 and using biochemical tests and phenotypic and agricultural characteristics to diagnose bacterial isolates and study sensitivity and resistance of bacterial isolates to ten antibiotics. if the results showed that the highest percentage of resistance was to the two antibiotics, Trimethoprim, and streptomycin, at 100%, while The least resistance was to the two antivirals Gentamycin and Rifampicin by 20%. In addition to Studying The sensitivity and resistance of bacterial isolates to four heavy Metals, namely Mercury chloride (HgCl), Cobalt chloride (CoCl), Cadmium Chloride (CdCl), and nickel chloride (NiCl). The resistance was high with Cadmium chloride salt (CdCl) and the lowest salt Mercury Chloride (HgCl). PCR techniques were used to diagnose samples based on 16S rRNA sequence analysis and comparison of sequences resulting from DNA amplification with standard isolates within NCBI to detect new bacterial isolates
Highlights
The leguminous family Leguminosae is known as the Bean family, and It is a major source for humans and animals
Placed in Order Laguminalse, under three families fall: Pcaescaeae, (Papilionaceae) [2]. The members of this family are distinguished from other plant families by forming a symbiotic relationship with Rhizobia, where this relationship resulted in the formation of root nodules and the fixation of atmospheric nitrogen and its conversion into ammonia that can be utilized by plants [3]
Polymerase Chain Reaction (PCR) primers for rhizobia species based on 16s rRNA sequence analysis and comparison with fixed data by DNA detection of species and shapes between strains of the same species using PCR technique to know and estimate the diversity of rhizobia and show ERIC and REP sequences are present in Rhizobia [13].The aims study to isolate the bacterial species belong to the genus Rhizobium from some leguminous plants in the some areas of Nineveh Governorate and to identify the isolated bacterial species using the microbiological, biochemica characteristics and their diagnosis at by using the specific PCR technique
Summary
The leguminous family Leguminosae is known as the Bean family, and It is a major source for humans and animals. The genera of the family Rhizobiaceae are Rhizobium and Allorhizobium and Mesorhizobuim and Bradyrhizobium and Sinorhizobium [4] Members of this family are organotrophic, aerobic and facultative [5], is rod-shaped, don’t from of spores, endemic to the soil and its members form root nodules that fix atmospheric nitrogen in the roots of legumes [6]. There are found in single or pairs and are motile by grow better growth ranges between (25-30o C) and many of they are unable (37o C) and they have the ability to benefit from a wide range of carbon compounds [7] These bacteria are collected inside the ganglia to form the so-called Bacteroids, which have the ability to fix nitrogen [8]. PCR primers for rhizobia species based on 16s rRNA sequence analysis and comparison with fixed data by DNA detection of species and shapes between strains of the same species using PCR technique to know and estimate the diversity of rhizobia and show ERIC and REP sequences are present in Rhizobia [13].The aims study to isolate the bacterial species belong to the genus Rhizobium from some leguminous plants in the some areas of Nineveh Governorate and to identify the isolated bacterial species using the microbiological, biochemica characteristics and their diagnosis at by using the specific PCR technique
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